A descriptive cross-sectional study was conducted among the immunization technicians in health centers of Al-Matama Locality, River Nile State, Sudan, during the year 2017. All of the technicians (20) in the immunization health centers in the locality were included (10 centers). Results revealed that 40% of the participants have experienced between 8-12 years and 90% of them found trained on the rotavirus vaccine. The participants knowledgeable on the rotavirus vaccine consist was 10% for a virus separated from its toxins, 15% for a genetically modified virus and 75% for a live attenuated virus. Likewise, their knowledgeable on the methods of prevention of rotavirus was 5% by washing hands, 5% avoiding overcrowding during the season of the spread of the disease, 30% vaccination using rotavirus vaccine and 60% that all mentioned are true. All of the participants found knowledgeable about the targeting age, besides most of them are aware of the number of vaccine doses, how it can be applied and the side effects. In conclusion, training the staff on the immunization program will improve their skills, increase their awareness and reduce the percentage of defaulters in the locality.
Keywords: Knowledge, technicians, rotavirus, Al-Matama Locality.